侧缘真龙虱提取物CL对多柔比星致慢性肾炎大鼠肾功能的保护作用

张晶娜, 杨志斌, 王梦如, 杨自忠, 董欣辛, 肖怀, 许凯歌, 张成桂

中国药学杂志 ›› 2019, Vol. 54 ›› Issue (15) : 1245-1252.

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中国药学杂志 ›› 2019, Vol. 54 ›› Issue (15) : 1245-1252. DOI: 10.11669/cpj.2019.15.010
论著

侧缘真龙虱提取物CL对多柔比星致慢性肾炎大鼠肾功能的保护作用

  • 张晶娜a, 杨志斌a,b,c, 王梦如a, 杨自忠a,b,c, 董欣辛a, 肖怀a,b,c, 许凯歌b, 张成桂a,b,c*
作者信息 +

Protective Effect of the Cybister lateralimarginalia Extract CL on Renal Function of Rats with Chronic Nephritis Induced by Doxorubicin

  • ZHANG Jing-naa, YANG Zhi-bina,b,c, WANG Meng-rua, YANG Zi-zhonga,b,c, DONG Xin-xina, XIAO Huaia,b,c, XU Kai-geb, ZHANG Cheng-guia,b,c*
Author information +
文章历史 +

摘要

目的 通过建立多柔比星诱导慢性肾炎模型及脂多糖诱导RAW264.7细胞炎症模型,研究侧缘真龙虱提取物CL对多柔比星慢性肾炎大鼠肾功能的保护作用及其抗炎作用。方法 采用一次性尾静脉注射多柔比星6 mg·kg-1建立慢性肾炎大鼠模型,造模2 w后,各组分别灌胃给予肾炎康复片、醋酸泼尼松、侧缘真龙虱提取物CL高、低剂量药物,正常对照组和模型对照组给予生理盐水。每天记录大鼠体重,每周末收集24 h尿液并测定尿蛋白含量;末次给药后,次日解剖大鼠,计算肾脏、肝脏指数,测定血清中血清总胆固醇(serum total cholesterol,TC)、甘油三酯(triglyceride,TG)、血清总蛋白(serum total protein,TP)、血清白蛋白(serum total protein,ALB)、血尿素氮(blood urea nitrogen,BUN)、血肌酐(serum creatinine,Scr)的含量,观察肾组织的病理变化;建立脂多糖(LPS,10 μg·mL-1)诱导RAW264.7细胞炎症模型,正常对照组与模型对照组给予PBS,其余各组分别给予侧缘真龙虱提取物CL低、中、高剂量药物,通过酶联免疫吸附法检测细胞上清液中肿瘤坏死因子(TNF)-α及诱导型一氧化氮合酶(iNOS)的表达量。结果 与正常对照组比较,模型对照组大鼠24 h尿蛋白含量,血清中TC、TG、BUN和Scr的含量,肾脏指数、肝脏指数显著升高(P<0.01或P<0.05),体重、TP和ALB显著降低(P<0.01或P<0.05);且LPS诱导RAW264.7细胞分泌促炎因子TNF-α和iNOS显著增加(P<0.01)。与模型对照组比较,肾炎康复片组、醋酸泼尼松组、侧缘真龙虱提取物CL各剂量组24 h尿蛋白含量,血清中TC、TG、BUN和Scr的含量均显著降低(P<0.01或P<0.05),体重、TP和ALB显著升高(P<0.01或P<0.05);肾组织病理情况得到一定的改善;且侧缘真龙虱提取物CL各剂量均能减少LPS诱导RAW264.7细胞分泌促炎因子TNF-α和iNOS(P<0.01)。结论 侧缘真龙虱提取物CL具有明显的抗炎作用,且对多柔比星慢性肾炎大鼠的肾功能具有良好的保护作用。

Abstract

OBJECTIVE To establish a model of chronic nephritis induced by doxorubicin and a model of RAW264.7 cell inflammation induced by lipopolysaccharide(LPS), and to study the protective effect of the Cybister lateralimarginalia extract CL on the renal function of rats with chronic nephritis induced by doxorubicin and its anti-inflammatory effect. METHODS A rat model of chronic nephritis was established by a single intravenous injection of doxorubicin (6 mg·kg-1). After 2 weeks of modeling, normal control group and model control group were given normal saline while other groups were given intragastric administration of CL high and low dose, Shenyankangfu tablets and prednisone acetate respectively. The body weight of the rats was recorded every day. The 24 h urine was collected every weekend and the urine protein content was measured. After the last administration, the rats were dissected the next day, and the kidney and liver indexes were calculated. The levels of TP, ALB, TC, TG, BUN and Scr in serum were determined. The pathological changes of kidney tissues were observed. The lipopolysaccharide (LPS 10 μg·mL-1) was used to induce the RAW264.7 cell inflammation model. PBS was given to the normal control group and the model control group while CL low, medium and high dose were given to other groups respectively. The levels of TNF-α and iNOS in the supernatant of cells were detected by enzyme-linked immunosorbent assay(Elisa). RESULTS Compared with the normal control group, the content of 24 h urine protein, the levels of TC, TG, BUN and Scr in serum, kidney index and liver index were significantly increased in the model control group (P<0.01 or P<0.05), but the body weight of rats, the levels of TP and ALB in serum were significantly decreased (P<0.01 or P<0.05). The secretion of pro-inflammatory factors TNF-α and iNOS were significantly increase in RAW264.7 cells with inflammatory induced by LPS (P<0.01). Compared with the model control group, the content of 24 h urine protein and the levels of TC, TG, BUN and Scr in serum were significantly decreased in the Shenyankangfu group, the prednisone acetate group and the CL group(P<0.01 or P<0.05), but the body weight of rats, the levels of TP and ALB in serum were significantly increased (P<0.01 or P<0.05); the pathological condition of renal tissue was improved to some extent. Each dose of CL could reduce the secretion of pro-inflammatory factors TNF-α and iNOS in RAW264.7 cells with inflammatory induced by LPS (P<0.01). CONCLUSION The extract of Cybister lateralimarginalia has an obvious anti-inflammatory effect and has a good protective effect on chronic nephritis in rats.

关键词

侧缘真龙虱 / RAW264.7细胞 / 抗炎作用 / 慢性肾炎

Key words

Cybister lateralimarginalia / RAW264.7 cell / anti-inflammatory effect / chronic nephritis

引用本文

导出引用
张晶娜, 杨志斌, 王梦如, 杨自忠, 董欣辛, 肖怀, 许凯歌, 张成桂. 侧缘真龙虱提取物CL对多柔比星致慢性肾炎大鼠肾功能的保护作用[J]. 中国药学杂志, 2019, 54(15): 1245-1252 https://doi.org/10.11669/cpj.2019.15.010
ZHANG Jing-na, YANG Zhi-bin, WANG Meng-ru, YANG Zi-zhong, DONG Xin-xin, XIAO Huai, XU Kai-ge, ZHANG Cheng-gui. Protective Effect of the Cybister lateralimarginalia Extract CL on Renal Function of Rats with Chronic Nephritis Induced by Doxorubicin[J]. Chinese Pharmaceutical Journal, 2019, 54(15): 1245-1252 https://doi.org/10.11669/cpj.2019.15.010
中图分类号: R285.5   

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基金

国家自然科学基金项目资助(81660605,81860742,81860765);云南省教育厅科学研究基金产业化培育项目资助(2016CYH15);大理大学青年教师科研基金项目资助(KYQN201535)
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